Composite

Part:BBa_K2996503:Design

Designed by: Shifeng Jiang   Group: iGEM19_SJTU-BioX-Shanghai   (2019-10-03)


The part is aimed to create a fused protein of C-luciferase and zincfinger


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 846
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 302
    Illegal AgeI site found at 441
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We used GGGGS-GGGGS linker to link the two proteins.The reverse primer of DNA of C-luciferase and the forward primer of DNA of DBD are the same in 15 base pairs,which ensures the result of overlapping PCR.


Source

E.coli

References